Wax soo wadaban
Hepes; CS No.:7365-45-9
Ereyo isku mid ah: 1- [[(2-hydroxyetyl) -1-piperazinyl] -1-piperazinyl] -1-piperazinyl] -1-sulfonicacidyl; aashito); n- (2-hydroxyetyl) piperazine-n'-2-ethanazulferey acid; n'-2-hydroxyetyl) acid-ka aashitada); n'-2-hydroxyetylpiperazine-n-2-ethanasulflonic acid; Tvz 7; wuxuu ahaa 13-piperazulletyl);
Hantida kiimikada ee Hepes
● Imaanshaha / Midabka: budada crystalline cad
Cadaadiska uumiga: 0pa 25 ℃
● dhibic dhalaalaysa: 234-238 ° C
● Index nooleeya la'aanta ah: n20 / d 1.339
● Meel karkaraya: 408 ℃ [101 325 p pa]
PKA: 7.5 (25 ℃)
Psa Psas:89.46000
● cufnaanta: 1.325 g / cm3
● Leein: -0.55930
● Kaydinta ku meel gaarka ah
SOLLEBLITLE.:H2O: 1 m at 20 ° C, cad, midab la'aan
● Xoolaha biyaha ..Sooluluble
● Xlogp3: -4.1
● Ku tabaruca degganaanshaha deganaanshaha hydrogen-ka tirinta: 2
● Aqbalida debaajiga hydrogen debaaji: 5
● Tixraac taxane ah oo la wareegi karo: 4
● sax ah tirada saxda ah: 238.09872833
● Tirinta culus ee atomka: 15
Isku-duwaha: 254
Macluumaadka SAFT
● Sawirada (yada):
Xi
● Koodhadhka Hazard: XI
● Hadalada: 36/37/38
● Hadafyada badbaadada: 24 / 25-22-36-36
Waxtar leh
Dhoola cadeynta canonical:C1cn (cc [nh +] 1ccs (= o) (= o) [O -]) CCO
Sharraxaad:Caspes waxaa lagu tilmaamay mid ka mid ah kuwa ugu wanaagsan ee ujeedooyinka ugu fiican ee loo heli karo cilmi baarista bayoolojiga. MADAXWEYNAHA BULKA, MOLLECULE-ga waa ZWitterionic, waxayna waxtar u leedahay sidii wax lagu dhejiyo ee Ph 6.8 illaa 8.2 (PKA 7.55). Waxaa caadi ahaan loogu isticmaalaa dhaqanka unugyada ee uruurinta 5mm illaa 30 mm. Caspes waxaa loo adeegsaday codsiyo badan oo kala duwan, oo ay ku jiraan dhaqanka unugyada. Waxaa badanaa loo isticmaalaa in lagu isticmaalo warbaahinta dhaqaalaha unugyada hawada. Hepes wuxuu adeegsadaa isticmaalkiisa ee ku saabsan tijaabooyinka vitro ee mg.
Adeegsiga:Unug aan sun ahayn. Waxaa loo istcimaalaa sidii biyo-ku-dhigga hydrogen ion, kaas oo xakamayn kara tirada guud ee PH ee muddo dheer. U fiirsashada waa 10-50mmmol / L. Guud ahaan, 20mmol / xiiran oo ku jira xalka nafaqada ayaa heli kara awooda waxtarka. Buffer bayoolojiyada. Hepes-ka waa wax lagu dhejiyo oo loogu talagalay cilmiga bayoolojiga, gaar ahaan loo isticmaalo dhaqanka unugyada si loo joogteeyo ph jir. Waxay u dhaqmaysaa qayb ka mid ah wax lagu dhifo, oo loo adeegsado diyaarinta weelasha. Waxaa lagu sifeeyay mid ka mid ah kuwa ugu wanaagsan ee loo yaqaan 'Ujeeddooyinka' ugu fiican ee loo adeegsado cilmi-baarista bayoolojiga.
Hordhac faahfaahsan
HantiWaxay u taagan tahay 2- (4- (2-hydroxyetyl) piperazin-1-yl) ethanies acid. Waa wakiil wax ku ool ah oo caadi ahaan loo isticmaalo cilmi baarista bayoolojiga iyo bayoolajiga. Hepes wuxuu ka caawiyaa sii wadidda deganaanshaha PH ee warbaahinta dhaqaalaha ee unugyada, maadaama ay awood u leedahay in labaduba aqbalo oo ay ku deeqaan protons, taas oo nidaamisa isbeddelada PH. Qaab-dhismeedka kiimikada iyo dhismaheeda ayaa si aad ah waxtar ugu leh dayactirka joogtada ah iyo fiiqsaadka fal-celinta bayoolojiga badan iyo falcelinta enzymatic. Caspes waxaa badanaa loo isticmaalaa dhaqanka unugyada, nadiifinta borotiinka, iyo farsamooyinka cilmiga bayoolajiga ee molikalal.
Isticmaal
Hepes wuxuu leeyahay codsiyo kala duwan oo ku saabsan beero cilmi baaris oo kala duwan. Qaar ka mid ah codsiyada islaamka ee hepes waa:
Dhaqanka unugyada: Caspes waxaa badanaa loo istcimaalaa inuu yahay qayb ka mid ah warbaahinta dhaqanka unugyada si loo ilaaliyo jawiga pon ee xasilloon. Waxay ka caawineysaa hagaajinta isbedelada PH ee ay sababaan nashaadaadka dheef-shiid kiimikaadka iyo uruurinta badeecadaha qashinka ee dhaqamada unugyada.
Enzyme assys:Hepes waxaa badanaa loo adeegsadaa wakiilka wax lagu dhejiyo ee enzyme damaanad ah inuu ilaaliyo PH-ga joogtada ah, kaasoo muhiim u ah shaqada saxda ah ee enzymes-ka. Waxay kaa caawineysaa xaqiijinta natiijooyinka saxda ah iyo dib-u-dhalinaya.
Electrophoresis:Caspes waxaa loo isticmaalaa inuu yahay wakiilka wax lagu dhejiyo ee farsamooyinka elektrophoris sida polyachiumidel gel electrophoresis (bogga) iyo agar jel electrophoresis. Waxay gacan ka geysaneysaa in la ilaaliyo tirada pH ee la rabo ee kala-goynta iyo falanqaynta qiimaha macromololecules sida borotiinka iyo acids nucleic.
Daahirinta borotiinka:Caspes marmarka qaarkood waxaa lagu daraa borotiinka si loo ilaaliyo pother-ka si loo ilaaliyo pH pH-da inta lagu gudajiro tillaabooyinka kala duwan ee nidaamka nadiifinta. Waxay gacan ka geysaneysaa dayactirka xasilloonida iyo dhaqdhaqaaqa borotiinka inta lagu jiro tallaabooyinka daahirinta.
Farsamooyinka cilmiga bayoolajiga ee molikalabka:Hepes waxaa loo isticmaali karaa farsamooyinka kala duwan ee cilmiga bayoolajiga ee molikalaligal, sida falcelinta silsiladaha polymarese) iyo dnna isku xigxiga, si loo ilaaliyo ph deggan. Waxay kaa caawineysaa kobcinta waxqabadka enzymes waxayna hubisaa natiijooyin sax ah oo lagu kalsoonaan karo.
Waxaa muhiim ah in la ogaado in ururinta gaarka ah ee hepes-ka oo codsigiisa ay ku kala duwanaan karaan iyadoo ku xiran xaaladaha tijaabada ah iyo shuruudaha.
