Alaabta
HEPES sodium cusbo
La mid ah: HEPES cusbo sodium; 75277-39-3; HEPES hemisodium cusbo; 103404-87-1; Sodium 2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonate; sodium 2-[4- (2-hydroxyethyl). )piperazin-1-yl]ethanesulfonate;HEPES (sodium); C8H17N2NaO4S; 4- (2-Hydroxyethyl) piperazine-1-ethanesulfonic acid hemisodium cusbo; 1-Piperazineethanesulfonic acid, 4- (2-hydroxyethyl) -, monosodium cusbo; ethanesulfonic acid sodium cusbo; 4- (2-hydroxyethyl) piperazin-1-ylethanesulphonate, UNII-Z9FTO91O8A; Z9FTO91O8A; sodium; 2-[4- (2-hydroxyethyl) piperazin-1-yl] ethanesulfonate; DTXSID4044458; CHEBI: 4675 278-169-7. cusbo,>=99.5% (titration);sodium hepes, N- (2-Hydroxyethyl) piperazine-N'- (2-ethanesulfonic acid) sodium cusbo; MFCD00036463; HEPES, cusbo sodium; HEPES sodium cusbo, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-O4-SNa xal cusbo; (1M) darajada;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-06108Asodium; N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES sodium cusbo, BioXtra,>=99.0% (titration);HEPES sodium cusbada, Vetec(TM) reagent darajada, 96%; W-104397; Q27120698; Sodium2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonate; 1-piperazineethanesulfonic acid, monosodium cusbo, N- (2-Hydroxyethyl) piperazine-N'- (2-ethanesulfonic acid) sodium cusbo; >> 99.5%
Hantida Kiimikada ee HEPES Sodium Salt
● Muuqashada/ Midabka: budo cad
● Cadaadiska Uumiga:0Pa at 25℃
● Meesha dhalaalaysa:234 °C
● PKA: 7.5 ( saacada 25 ℃ )
● PSA：92.29000
● Cufnaanta:1.504[20℃]
● LogP:-0.90190
● Heerkulka Kaydinta: Ku kaydi RT.
● Xasaasi ah: Hygroscopic
● Solubility
● Biyuhu milmayn: Waxay ku milmeen biyaha.
● Tirada ku-deeqayaasha curaarta Hydrogen:1
● Tirada Aqbalaada Debaajiga Hydrogen:6
● Tirada curaarta wareega:5
● Mass Sax ah:260.08067248
● Tirada Atomka Culus:16
● Kakanaanta:272
Macluumaadka Badbaadada
● Sawiro:
● Xeerarka halista:
● Bayaanada Badbaadada:22-24/25
Faa'iido leh
Fasalada Kiimikada:Isticmaalka Kale -> Biological Buffers
Dhoola-cadaynta Canonical:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Adeegsada:Xalka cusbada HEPES sodium wuxuu ku habboon yahay daraasadaha dhaqanka unugyada. Waxa loo isticmaali karaa diyaarinta xal-u-helka HEPES. Waxa loo isticmaali karaa sidii kayd inta lagu jiro qiimaynta fosforyaalka ee fibroblasts-ka-daran. Waxa laga yaabaa in loo isticmaalo in lagu diyaariyo sifaynta adag ee sifaynta, loo isticmaalo go'aaminta thyroxin bilaashka ah ee serum by radioimmunoassay. Xalka cusbada HEPES sodium wuxuu ku habboon yahay in loo isticmaalo daraasadaha dhaqanka unugga. Waxa loo isticmaali karaa diyaarinta xal-u-helka HEPES. Waxa laga yaabaa in loo isticmaalo baffer ahaan inta lagu jiro qiimaynta fosforyaalka ee fibroblasts-ka-daran. Waxa laga yaabaa in loo isticmaalo in lagu diyaariyo sifaynta adag ee sifaynta, loo isticmaalo go'aaminta thyroxin bilaashka ah ee serum by radioimmunoassay.
Hordhac faahfaahsan
HEPES sodium cusbo, oo sidoo kale loo yaqaan 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium sodium, waa nooc caadi ahaan loo isticmaalo ee HEPES. Waa iskudhis organic zwitterionic ah oo u dhaqma sidii wakiilka buffering ee cilmi bayooloji iyo bayooloji.
HEPES sodium cusbada waxaa inta badan loo isticmaalaa warbaahinta dhaqanka unugyada iyo bayooloji baffers ay sabab u tahay awoodda ay u leedahay in la ilaaliyo kala duwanaanta pH deggan agagaarka xaaladaha jireed (pH 7.2 - 7.6). Aad bay ugu milmay biyaha oo waxay leedahay suntan xad-dhaaf ah, taasoo ka dhigaysa mid ku habboon codsiyada kala duwan.
Nooca cusbada sodium ee HEPES ayaa doorbidaya codsiyo badan maadaama ay kor u qaadayso milanka iyo xasilloonida, marka la barbar dhigo qaabka aashitada bilaashka ah. Waxaa ganacsi ahaan loo heli karaa sida budada crystalline cad oo si fudud loogu milmi karo biyaha si loogu diyaariyo xalalka shaqada.
Cilmi baadhayaashu waxay caadi ahaan u isticmaalaan cusbada HEPES sodium ee farsamooyinka bayoloji molecular, dhaqanka unugga, imtixaannada enzymes, nadiifinta borotiinka, iyo tijaabooyinka kale ee kiimikaad ee kontoroolka pH ay muhiim u tahay. Awooddeeda wax-ka-qabashada iyo la jaan-qaadka hababka noolaha ayaa ka dhigaya doorasho caan ah oo lagu ilaalinayo xaaladaha ugu habboon ee dhaqdhaqaaqa iyo xasilloonida unugyada noolaha ee kala duwan.
Kahor intaadan isticmaalin cusbada soodiyamka ee HEPES codsi kasta oo gaar ah, waxaa lama huraan ah in dib loo eego suugaanta, la tashado talooyinka alaab-qeybiyeyaasha, oo la wanaajiyo fiirsashada iyo kala duwanaanta pH ee ku saleysan shuruudaha tijaabada ee gaarka ah.
Codsiga
HEPES sodium cusbadu waxay ka heshaa codsi baahsan oo laga helo aagag cilmi-baaris oo kala duwan oo bayooloji iyo bayooloji ah. Qaar ka mid ah adeegsigeeda muhiimka ah waxaa ka mid ah:
Dhaqanka Unugga:HEPES sodium cusbada waxaa sida caadiga ah lagu daraa warbaahinta dhaqanka unug si loo ilaaliyo pH deggan gudaha xadka jireed iyo in la siiyo jawi ku haboon unugyada si ay u koraan oo ay u bataan.
Wakiilka kaydinta:HEPES sodium cusbada waxaa inta badan loo shaqeeyaa sidii wakiilka kaydiya ee kaydinta bayoolojiga iyo xalalka, oo ay ku jiraan tijaabooyinka enzyme, nadiifinta borotiinka, iyo farsamooyinka bayoolaji molecular. Waxay ka caawisaa in ay joogteyso pH joogto ah iyada oo iska caabinaysa isbeddelada aashitada ama alkalinity.
Electrophoresis: HEPES sodium cusbada waxaa loo isticmaalaa sida kaydinta jel electrophoresis si loo ilaaliyo pH deggan inta lagu jiro kala soocida asiidhyada nucleic ama borotiinada. Waxay ka hortagtaa isbeddellada pH ee saameyn kara socdaalka iyo qaababka kala-soocidda molecules ee jelka.
Xasiloonida Barootiin: HEPES sodium cusbo ayaa lagu daraa xalalka borotiinka si loo dejiyo qaab-dhismeedkooda loona ilaaliyo hawlahooda. Waxay ka caawisaa ilaalinta deegaanka ugu fiican ee pH ee lagama maarmaanka u ah xasiloonida borotiinka iyo shaqeynta.
Waxqabadka Enzyme: HEPES sodium cusbada waxaa loo isticmaalaa sidii wax u kaydiya falcelinta enzymatic si loo ilaaliyo pH-ga la rabo ee hawsha enzyme ugu fiican. Waxay kaa caawinaysaa in la hubiyo in ensaymyadu u shaqeeyaan waxtarkooda ugu sarreeya iyo saxnaanta.
Sawirka Unugga Tooska ah:HEPES sodium cusbada ayaa inta badan lagu daraa warbaahinta sawir-qaadista ee tijaabooyinka sawirka unugyada nool. Awoodeeda kaydintu waxay caawisaa ilaalinta pH-ga la rabo waxayna ka hortagtaa isbedbedelka saameeya jiritaanka iyo fluorescence unugyada.
Farsamooyinka Bayolojiga Molecular: HEPES sodium cusbada waxaa si weyn looga shaqeeyaa farsamooyin bayoloji molecular oo kala duwan, oo ay ku jiraan DNA ama RNA go'doomin, PCR, taxanaha DNA, iyo falanqaynta borotiinka. Awoodeeda wax-ka-qabashada waxay u oggolaanaysaa natiijooyin sax ah oo la isku halayn karo inta lagu jiro habraacyadan.
Waxaa muhiim ah in la ogaado in codsiga gaarka ah iyo xoogga saarista cusbada sodium ee HEPES ay kala duwanaan karto iyadoo ku xiran shuruudaha tijaabada iyo nidaamka bayoolojiga ee daraasadda lagu hayo. Sidaa darteed, waxaa habboon in lagala tashado suugaanta iyo talooyinka alaab-qeybiyaha si loo isticmaalo sida ugu habboon.
