Wax soo wadaban
Hepps; CS No.: 16052-06-5
Ereyo isku mid ah: Hepps; 16052-06-5; Epps; 4- (2-hydroxyetyl) -1-piperrazineproponic acid; aashito; Aashitada 240-198-8; BRN 3957385; 8jn5pq99c; aashito; ep1; n-2-hydroxyetylpiperpiperazine-n'-3-popanesulfonive acid; UNI-8JN5PQW99CMBL984003; 42298; EPPs;> = 99.5% (TITRAING) 99%; AC-24630; as-69273; epps, bioxtra,> = 99.5% . Sulfonic acid; n-2-hydroxyetythylpiperpiperazine-n-3-preaniesulfonic aashito; Q1563525; w-107779; aashito; (Hepps);
Hantida kiimikada ee Hepps
● Imaanshaha / Midabka: budada crystalline cad
● dhibic dhalaalaysa: 237-239 ° C (iftiin.)
● Index nooleenta ah: 1.537
PKA: 8.0 (25 ℃)
Psa Psas:89.46000
● cufnaanta: 1.285 g / cm3
● Leein: -0.16920
● Kaydinta ku meel-gaarka ah ee kaydhka rt.
SOLLEBLITLE.:H2O: 1 m at 20 ° C, cad, midab la'aan
Wadahada biyaha. 25.2 g / l (20 ° C)
● Xlogp3: -3.6
● Ku tabaruca degganaanshaha deganaanshaha hydrogen-ka tirinta: 2
● Aqbalida debaajiga debaajiga ah: 6
● Tirada debaajiga la wareegi karo: 6
● sax ah tiro: 252.114337830
● Tirinta culus ee atomka: 16
Isku-duwaha: 280
Macluumaadka SAFT
● Sawirada (yada):
Xi
● Koodhadhka Hazard: XI
● Weedhaha: 36/37 / 38-22
● Hadafyada badbaadada: 24 / 25-36-26
Waxtar leh
Fasalada kiimikada:Xerooyinka nitrogen -> piperrazines
Dhoola cadeynta canonical:C1cn (ccn1ccccs (= o) (= o) o) cco
Adeegsiga:EPPs ma badanaa la isticmaalaa wax fiican? Waxaa badanaa loo isticmaalaa inuu yahay kala-sooca ee u dhexeeya Ulthathathin isoelectric diiradda saareynta gels iyo kor u qaadida xallinta fosphoglomatase. Waxaa loo istcimaalaa sida wakiilka wax lagu dhejiyo ee bayoolajiga iyo bayoolajiga.
Hordhac faahfaahsan
EPPS waxay u taagan tahay N- (2-hydroxyetyl) piperazine-n '- (3-preapanansulic acid). Waa wakiilka loo yaqaan 'zwitfferional' oo ah in loo isticmaalo cilmiga bayoolojiga iyo cilmi baarista bayoolaji.ehere waa qaar ka mid ah qodobbada muhiimka ah ee ku saabsan EPPS:
Dhismaha guryaha:EPPs waa wakiil wax ku ool ah oo wax ku ool ah oo ka caawiya joogtaynta safka si joogto ah ee dejinta tijaabada. Waxay leedahay qiimaha PKA ee ah 8.09, oo ka dhigaya mid ku habboon inuu ku soo celiyo tirada pH ee 7.0-9.0.
Deganaanshaha:EP--yada waxay soo bandhigtay xasillooni wanaagsan oo heerkul badan oo kala duwan oo ay ku yar tahay isbeddelada heerkulka marka loo eego wakiilada kale ee soo-saarka sida Tris iyo Phosphate Buthotes. Deganaanshani waxay ka dhigeysaa inay ku habboon tahay in lagu isticmaalo baaritaanka bayoolajiga iyo cilmiga bayoolajiga ee u baahan xaaladaha xaruunta ee PH.
Codsiyada bayoolojiga:EPPs waxaa badanaa loo isticmaalaa daraasado bayooloji ah, sida nadiifinta borotiinka, enzyme assys, iyo DNA / DNA-da. Awoodda wax u dhimaya iyo xasilloonida ph jirdhiska ah waxay qiimo u leedahay codsiyadan.
Dhaqanka unugyada:EPP-yada waxaa sidoo kale loo isticmaali karaa wakiilka wax lagu keydiyo ee warbaahinta dhaqanka ee unugyada. Waxay gacan ka geysaneysaa in la ilaaliyo jawi deggan oo xasilloon oo u qalma koritaanka iyo dayactirka noocyada unugyada qaarkood.
Ion iyo iswaafajinta:EPPs waa xarun zwitterteronic, taasoo la micno ah inay kujirto khidmad togan iyo kuwa xunba. Muuqaalkaani wuxuu u oggolaanayaa inuu si wax ku ool ah u xajisto labadaba acidic iyo xalka aasaasiga ah. Waxay la jaan qaadayaan moleekulo bayooloji oo kala duwan waxaana lagu yaqaanaa faragalin yar oo ku saabsan falcelinta enzymatic.
Markaad isticmaaleyso EPPS, waxaa muhiim ah in la raaco tilmaamaha ku taliyay iyo Tilmaamaha PH ee lagu taliyay ee soo saaraha soo saaraha. Intaa waxaa sii dheer, had iyo jeer jimicso si taxaddar leh oo raac hab-hagitaanada ku habboon ee amniga marka aad wax ka qabanayso wakiil kasta oo kiimiko ah ama xayawaanadeed.
Isticmaal
Hepps (n- (2-hydroxyetyl) piperazine-n'-3-peipanine-n'fiesulfonic acid) waa xaraashka zwitterteronic-ka kaas oo badanaa loo isticmaalo cilmibaadhista bayoolojiga iyo cilmi baarista bayoolaji. Codsiyada qaarkood waxaa ka mid ah:
Dhaqanka unugyada: Hepps waxaa loo isticmaalaa inuu yahay wakiilka wax lagu dhejiyo ee warbaahinta gacanta ee loo yaqaan 'Media' ee warbaahinta unugyada si loo ilaaliyo si joogto ah oo ay u siiso jawi ku habboon koritaanka unugyada iyo kordhinta. Waxay gacan ka geysaneysaa xasilinta PH gudaha jir ahaaneed oo ka fogow wixii saameyn xun ee ku saabsan geedi socodka gacanta.
Enzyme assys:Hepps waxaa badanaa loo isticmaalaa sidii wax lagu dhejiyo oo ku yaal enzyme damaanad ah oo lagu hagaajinayo shuruudaha PH ee looga baahan yahay waxqabadka ugu badan ee enzyme ugu badnaan. Awooda wax soo dhigga waxay ka caawineysaa in la ilaaliyo meel damci ah, oo hubinaya cabbiraadaha enzyme ee saxda ah iyo kuwa la isku halleyn karo.
Daahirinta borotiinka:Hepps waxaa loo isticmaali karaa inuu yahay wax ku dhejinaya hanaannada nadiifinta borotiinka. Waxay gacan ka geysaneysaa dayactirka xasilloonida iyo dhaqdhaqaaqa borotiinnada inta lagu gudajiro tillaabooyinka lagu nadiifiyo, sida chromatography compant ama nadiifinta borotiinka ah iyadoo la adeegsanayo sumcadaha shirkadda.
Electrophoresis:Hepps mararka qaarkood waxaa loo isticmaalaa sidii wax loo yaqaan '' '' Awooda 'PH PH' waxay ka caawineysaa sii wadidda xasilloonida inta lagu guda jiro orodka elektrophoratatic-ka, iyagoo ka caawiya kala sooca saxda ah ee moleekules.
Farsamooyinka cilmiga bayoolajiga ee molikalabka:Hepps waxaa loo isticmaali karaa sidii wax lagu dhejiyo ee ku jira farsamooyinka kala duwan ee molikaliqulolo bayoolajiga ee molikalalal, oo ay kujirto PCR (falcelinta silsiladaha polymarese), isku xirka DNA-da, iyo jawaab-celinta encymatic. Awoodda ay ku dhisan tahay waxay xaqiijineysaa xaaladaha wanaagsan ee PH ee Falf-celinta guuleysta iyo waxtarka leh.
Daraasadaha ku lug leh borotiinka xuubka:Hepps wuxuu si fiican ugu habboon yahay barashada borotiinka xuubka ee xuubka xuubka xuubka ah ee xuubka xuubka. Waxaa badanaa loo isticmaalaa sidii wax lagu dhejiyo ee tijaabooyin ku lug leh astaamaha shaqada ama dib-u-dejinta borotiinnada xuubka.
Waxaa muhiim ah in la ogaado in feejignaanta ugu habboon iyo pH ee Heppsku ay ku kala duwanaan karaan iyadoo ku xidhan codsiyada gaarka ah ama xaaladaha tijaabada ah. Sidaa darteed, waxaa lagugula talinayaa inaad kala tashato suugaanta ama soo-ururinta talooyinka ee loo adeegsado isticmaalka habboon iyo uruurinta Hepps tijaabooyinkaaga.
